Modifications: Adjust pH of medium after autoclaving to 7.4 using a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere.
Growth observed:
Growth conditions: anaerobic 37 °C
Modifications: Adjust pH of medium after autoclaving to pH 7.3 - 7.5 using a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere.
Growth observed:
Growth conditions: anaerobic 37 °C